Home|Journals|Articles by Year Follow on Twitter

Directory for Medical Articles

Open Access

Original Article

In-house preparation of pentavalent 99mTc labeled dimercaptosuccinic acid [99mTc(V)DMSA], its quality control and clinical applications

Hameed Ullah, Syed Qaiser Shah, Aakif Ullah Khan, Shakil Ahmad, Muhammad Rauf Khattak.

Background: A convenient method was developed for the preparation of pentavalent 99mTc dimercaptosuccinic acid using a commercially available meso-2,3-dimercaptosuccinic acid (DMSA) kit. The effects of various parameters, including incubation time, pH, and concentration of sodium bicarbonate on radiochemical purity (RCP) yield were evaluated. To determine tri- and pentavalent fractions, RCP yield of the labeled DMSA was measured using different solvent systems. Methods: The RCP yield was studied using a thin-layer chromatography on silica gel as the stationary phase and n-butanol/acetic acid/distilled water (3:2:2) as the mobile phase. Results: It was observed that hydrolyzed 99mTcO2 and 99mTc(III)-DMSA remained at the origin (Rf = 00.1), and 99mTc(V)-DMSA at (Rf = 0.50.6), while free pertechnetate, 99mTcO−4 moved to the solvent front (Rf = 0.91). However, in acetone, 99mTc(III)-DMSA, 99mTc(V)DMSA, and hydrolyzed 99mTcO2 stayed at the origin (Rf = 00.1), while free pertechnetate, 99mTcO−4 moved to the solvent front (Rf = 0.91). When developed with 5% glycine, 99mTcO2 stayed at the origin and the other species migrated to the solvent front. The labeling efficiency of the 99mTc(V)DMSA was 96.6 0.5% and was stable for 24 hours at room temperature. Conclusion: Higher labeling yield, easy preparation with low cost, and good stability make 99mTc(V)-DMSA, a promising radio drug for clinical use.

Key words: Medullary thyroid cancer, 99mTc(V)-DMSA, radiochemical purity, thin-layer chromatography.

Similar Articles

Inhibition of CCL7 derived from Mo-MDSCs prevents metastatic progression from latency in colorectal cancer.
Ren X, Xiao J, Zhang W, Wang F, Yan Y, Wu X, Zeng Z, He Y, Yang W, Liao W, Ding Y, Liang L
Cell death & disease. 2021; 12(5): 484

Novel silver glycinate conjugate with 3D polymeric intermolecular self-assembly architecture; an antiproliferative agent which induces apoptosis on human breast cancer cells.
Banti CN, Raptopoulou CP, Psycharis V, Hadjikakou SK
Journal of inorganic biochemistry. 2021; 216(): 111351

Metabolic classification of bladder cancer based on multi-omics integrated analysis to predict patient prognosis and treatment response.
Tang C, Yu M, Ma J, Zhu Y
Journal of translational medicine. 2021; 19(1): 205

State of knowledge: Histolocalisation in phytochemical study of medicinal plants.
El Babili F, Rey-Rigaud G, Rozon H, Halova-Lajoie B
Fitoterapia. 2021; 150(): 104862

A protein-sulfosalicylic acid/boswellic acids @metal-organic framework nanocomposite as anticancer drug delivery system.
Özsoy M, Atiroğlu V, Guney Eskiler G, Atiroğlu A, Deveci Ozkan A, Özacar M
Colloids and surfaces. B, Biointerfaces. 2021; 204(): 111788

Full-text options

Add your Article(s) to Indexes
• CiteIndex: Articles & Statistics

American Journal of Diagnostic Imaging


Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.