Proprietary Herbal Extract Downregulates the Gene Expression of Il-1α in HaCaT Cells: Possible Implications Against Nonscarring Alopecia
Erkin Pekmezci, Cihat Dundar, Murat Turkoglu.
Abstract
Background: Currently while, topical minoxidil and oral finasteride are the only medications approved in androgenetic alopecia (AGA), the cause oriented treatment and immunsupressive treatment are being performed in telogen effluvium (TE) and alopecia areata (AA) respectively. Considering the inflammatory factors in the pathogenesis of these three nonscarring alopecia forms, we have formulated a mixture for topical usage composed of six different herbal extracts (HE) which have already known antiinflammatory and antioxidant features. Materials and Methods: In addition to performing the phytochemical analysis of HE, we detected the gene expression level of IL-1α, the crucial hair loss mediator, for the putative efficacy in nonscarring alopecia. Cell proliferation assay was performed by XTT reagent. After determination of non-cytotoxic concentration, HaCaT cells were treated with HE. RNA isolations were carried out from both non-treated and treated cell groups by using TRI-reagent. Gene expressions of IL-1α and as control GAPDH were determined by RT-qPCR analysis. Results: Results were represented as IL-1α/GAPDH Fold Change. HE solution caused statistically significant downregulation of IL-1α gene expressions (p
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