Abstract

This study aims to evaluate the potential of endophytic fungi isolated from Gandaria (Bouea macrophylla Griffth) as sources of enzyme inhibitor, antibacterial, antioxidant and identify the chemical compounds of active extract. The endophytic fungi was identified as a fungi closely related to the genus Neofusicoccum ribis based on sequence analysis of the 28S rDNA and ITS region. N. ribis was cultured on potato dextrose broth (PDB) and extracted with hexane, ethyl acetate, and partition between methanol and ethyl acetate, respectively. Bioactivity screenings as enzyme inhibitor (acetylcholinesterase/ AChE and β-glucosidase), antibacterial and antioxidant were conducted by thin layer chromatography (TLC) - bioautography method. The chemical compounds were analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). The results showed that the ethyl acetate extract of N. ribis had the activity as enzyme inhibitor for AChE and β-glucosidase. The MIC values of N. ribis extract against Staphylococcus aureus and Escherichia coli were in the range of 512->512 mg/mL. Hexane extract had better antioxidant activity (IC50= 479.66 µg/mL) than ethyl acetate extract (IC50 = 928.09-1102.60 µg/mL). The GC-MS analysis showed 12 identified chemical compounds were found in the ethyl acetate extract of N. ribis.

Key words: endophytic fungi, N. ribis, enzyme inhibitor, antibacterial, antioxidant