Aim: To clarify an effect of xanthones on inflammation status in hypercholesterolemic rats.
Method: A total of 32 Wistar rats were divided into four groups (n=8), including control, hypercholesterolemic diet groups, hypercholesterolemic diet + xanthone at dose 35; 70; and 140 mg/kg body weight (mg/kgBW). Control group received standard diet for 60 days. Hypercholesterolemic diet group received standard diet plus yellow egg, sheep oil, cholic acid, and pig oil for 60 days per oral. Analysis of nuclear factor-kappa beta p65/p50 distribution and tumor necrosis factor-alpha level, was done using enzyme linked immunosorbent assay technique. Analysis of nitric oxide level was done by colorimetric technique using spectrophotometer.
Results: Hypercholesterolemic diet significantly increased nuclear factor-kappa beta p65/p50 distribution, tumor necrosis factor-alpha level, and nitric oxide level compared with the control group (p
Key words: Xanthone; inflammation; NF-kB p65; TNF-a; NO; hypercholesterol diet
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