Abstract

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a notorious pathogen responsible for a significant portion of healthcare-associated infections (HAIs). Although clinical environments such as wards and surgical units are routinely monitored for MRSA contamination, non-clinical settings like administrative offices are often neglected. These areas, frequently used by staff and visited by outsiders, can serve as potential reservoirs for pathogenic microorganisms, especially MRSA. Objective: This study aimed to assess the extent of MRSA contamination in administrative offices of Bichi Emirate Specialist Hospital and to evaluate cleaning and disinfection practices among administrative staff. Methods: A cross-sectional study was conducted involving 21 administrative offices. Swab samples were collected from three commonly touched surfaces—keyboards, mice, and desks. These samples were subjected to standard bacteriological culturing techniques for isolation of S. aureus. Polymerase Chain Reaction (PCR) analysis was conducted to confirm methicillin resistance through detection of the mecA gene. Additionally, structured questionnaires were distributed to assess cleaning habits and awareness among staff. Results: A substantial number of the sampled surfaces showed contamination with S. aureus, and several were confirmed to be MRSA-positive through PCR. Most staff members reported infrequent cleaning practices, with minimal use of disinfectants and a general lack of awareness about MRSA transmission in non-clinical areas. Conclusion: The findings suggest that administrative offices may significantly contribute to nosocomial MRSA transmission. Integrating these areas into hospital-wide infection control programs is crucial for reducing microbial risks in healthcare facilities.

Key words: MRSA, Hospital Environment, Administrative Offices, mecA gene, PCR, Infection Control