Black galingale (Curcuma aeruginosa Roxb.) is an important medicinal plant that is widely distributed in South and South-east Asia, however utilities in Indonesia is still limited due to superior varieties and seedling availability. Micro-propagation could provide rapid and true to type seedlings to meet the black galingale demand. The objectives of this study were to develop the best in vitro medium composition for shoot multiplication and to compare the field growth of plants originated from the rhizome and in vitro planlet. The in vitro experiment was arranged in randomized complete block design with two factors and five replications. The first factor was BAP concentration consisted of 0, 2, 4, 6, and 8 mg L-1. The second factor was IAA concentration consisted of 0, 0.5, and 1.0 mg L -1. Our results showed that number of shoot, number of leaves and plantlet height were significantly affected by the interaction of IAA and BAP. The highest number of shoots was obtained in the medium containing 6 mg L-1 BAP with no IAA. The in vitro grown plantlets were acclimatized and grown in the field. The growth of these in vitro originated plants was compared with those propagated through the rhizome.
Key words: shoot, propagation, in vitro, BAP, IAA
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