Fully automated urine analyzers are generally preferred in laboratories with high throughput. When these systems are used; especially the sediment analysis should be made by a well-skilled technician before reporting to discriminate well the interferences (like yeast or crystals) from erythrocytes and leukocytes. The purpose of the present study is to detect the correlation of erythrocyte and leukocyte numbers without any corrections in sediment field images of BT Uricell 1280 (BT products, İzmir, Turkey) fully automated urine analyzer with manual microscopy reports. A total of 528 first morning urine samples were studied by both BT Uricell 1280 and manual microscopy concurrently. The degree of concordance (Kappa coefficient) were evaluated. The sensitivity, specificity and positive and negative predictive value for the BT Uricell 1280 compared to manual microscopic examination were assessed. Precision and carry over studies were also performed. The degree of concordance of erythrocyte and leukocyte counts in microscopy of 528 urine specimens with manual microscopy was found to be 0.42 and 0.52 respectively (kappa coefficient). The sensitivity and specificity values of RBC and WBC were calculated for BT Uricell 1280 as 62.6%, 91.1%, 77.6%, 88.8%, respectively. The results of carry over analysis for both RBC and WBC were 0 %. The sediment microscopy analysis of erythrocytes and leukocytes with BT Uricell 1280 without any corrections presented moderate correlation with manual microscopy. A better correlation can be achieved by a more detailed examination of field images of the analyzer.
Key words: Urine microscopy, erythrocytes, leukocytes
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