Abstract

The present study emphasizes on synthesis of metallic silver nanoparticles from cell free supernatant of Aspergillus awamori strain KGSR12. The phase purity, composition, size and shape of the as synthesized nanoparticles were characterized using various analytic spectroscopic techniques including X-ray diffraction (XRD), Scanning electron microscope (SEM), UV–Visible (UV-Vis) and Fourier transform infrared (FT-IR) spectroscopy. Based on the SEM analysis, the particles are uniformly distributed and size is estimated to be 40-50 nm. Antibacterial activity of nanoparticles against significant human pathogens was conferred with well diffusion assay revealed strains Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae and Bacillus cereus. Thus the study concludes with biogenic and ecofriendly route for synthesizing silver nanoparticles with antibacterial activity against clinically important pathogens and attributes growing interest on fungi as an emerging source for synthesis of nanoparticles.

Key words: bioreduction, silver nanoparticles, Aspergillus awamori, antibacterial activity, FTIR