Treated Plasmodium berghei infected pregnant mice by Andrographis paniculata tablet (AS201-01) decreasing the TLR-4 expression and apoptosis index of placental tissue
Budi Prasetyo, Diantri Nari Ratih, Yustinasari, Hilkatul Ilmi, Lidya Tumewu, Aty Widyawaruyanti.
Abstract
Objective
This study aimed to evaluate the effect of Andrographis paniculata tablet (AS201-01) in decreasing expression of Toll-Like Receptor-4 (TLR-4) and apoptosis index of placental tissue of Plasmodium berghei infected pregnant mice.
Material and methods
A total of 24 pregnant mice were divided into 4 groups, G1 was uninfected pregnant mice group, G2 was untreated Plasmodium berghei infected pregnant mice, G3 was P. berghei infected pregnant mice treated with AS201-01 tablet and G4 was P. berghei infected pregnant mice treated with dihydropiperaquine-phosphate (DHP) tablets. All mice were terminated at day 15th of pregnancy. The placenta was collected. The TLR-4 expression and apoptosis index were evaluated using immunohistochemistry assay.
Results
The TLR-4 expression and apoptosis index on G3 was lower than G2 and showed a statistically significant difference (P = 0.00). The TLR-4 expression and apoptosis index of placental tissue on P. berghei infected pregnant mice which treated with AS201-01 tablet were decreased compared to untreated group and DHP tablet treated group as well.
Conclusion
AS201-01 tablet containing ethyl acetate fraction of A. paniculata could decrease TLR-4 expression and apoptosis index of placental tissue on P. berghei infected pregnant mice.
Key words: TLR-4 expression, Apoptosis index, Plasmodium berghei, in vivo assay, Andrographis paniculata tablet (AS201-01)
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