Endophytic fungi are a group of fungi which grow inside plant tissue without produce negative symtomps and able to produce biological active substances. This research was carried out to evaluate antibacterial and antioxidant activity of forty isolates of endophytic fungi which were isolated from 10 species of medicinal plants collected from Palolo, Central Sulawesi. Thin layer chromatography (TLC) bioautography-guided screenings were done to evaluate antibacterial and antioxidant activities. The antibacterial activity was done against Staphylococcus aureus InaCC B5 and Escherichia coli InaCC B4, while antioxidant activity was assessed by (2,2-diphenyl-1-picrylhydrazyl) DPPH method. The active extracts were further evaluated for their minimum inhibitory concentration (MIC) and half maximal inhibitory concentration (IC50) values by microdilution broth method. The result of TLC bioautography screening showed 30 extracts inhibited the growth of S. aureus, 29 extracts inhibited the growth of E. coli, 27 extracts inhibited both S. aureus and E. coli, and 23 extracts posessed antioxidant activity. There were 6 extracts that have MIC value < 100 µg/ml against S.aureus and 9 extracts with MIC value < 100 µg/ml against E coli. Six extracts indicated strong antioxidant activity.
Key words: antibacterial, antioxidant, endophytic fungi, bioautography
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