Antibiotic resistance is one of many serious health problems that has emerged both in developed and advanced countries in the world. One of many examples of antibiotic resistance is Mycobacterium tuberculosis resistance to standard therapy for tuberculosis. Alternative treatments have been done to overcome Mycobacterium tuberculosis resistance. Developing new compound or supplement from natural product is widely explored. Pomegranate fruit (Punica granatum L) has been used traditionally as an antimicrobial agent. However, toxicity of compounds should be investigated prior to development. This research was aimed to determine LC50 of the extract zebrafish (Danio rerio) embryos and to predict toxicity of compound in various parameters. Fish embryo acute toxicity test was done by using zebrafish (Danio rerio) embryo as subject to obtained safety characteristics of extract. Toxicities of compounds in pomegranate were predicted using software ADMET Predictor 7.1. Pomegranate peel ethanol extract revealed LC50 of 196,037 ± 9,2 µg/mL. Based on aquatic toxicity classification by OECD, pomegranate peel ethanol extract was classified as safe. Toxicity prediction showed that brevifolin was the safest substances among the other substances that contained in ethanol extract of pomegranate peel.
Key words: pomegranate peel, acute toxicity test, tuberculosis, molecular
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