Abstract

Objective: To determine the frequency of antibiotic resistance in pneumonia against fluoroquinolones.
Methodology: This prospective observational study included a sample size of 100 sputum and blood samples collected from Medicare and Cardiac General Hospital, as well as Sohail Trust Hospital. Karachi, Pakistan. The samples were tested for culture and sensitivity, and the antibiotic susceptibility pattern was recorded. The fluoroquinolone-resistant pneumonia was tested by the Kirby-Bauer disc diffusion technique. The fluroquinolone resistant sputum and blood samples were used to identify the presence of QRDR genes (gyrA, gyrB) was screened by polymerase chain reaction (PCR) amplification.
Results: The frequency of sensitivity of isolates to fluoroquinolones was 75%, and resistance was 25%. Among isolates, PCR analysis revealed a statistically significant association between fluoroquinolone resistance and the presence of gyrA (p

Key words: fluoroquinolones, antibiotic resistance, hospital acquired pneumonia, polymerase chain reaction (PCR) amplification.