Home|Journals|Articles by Year Follow on Twitter

Directory for Medical Articles

Open Access

Original Article

Distilled liquid smoke coconut shell attenuates the cytokine profile of macrophages in oral ulcer in experimental model of diabetes mellitus

Meircurius Dwi Condro Surboyo, Diah Savitri Ernawati, Desiana Radithia, Bagus Soebadi, Fatma Yasmin Mahdani, Nurina Febriyanti Ayuningtyas, Fiona Cherrilia Adji, Novia Ambar Larasati.

The effects of distilled liquid smoke coconut shell (DLS-CS) on the macrophages and nuclear factor erythroid2-related factor 2 (Nrf2) and cytokine profiles, that is, tumor necrosis factor α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β), were analyzed in oral ulcer tissues affected by diabetes mellitus. Wistar rats were injected with alloxan to create diabetes mellitus, and an oral ulcer was made in the inferior labial fornix mucosa. The rats were treated with DLS-CS 20 l/20 g body weight, topically, once a day for 3, 5, and 7 days. The Nrf2, TNF-α, IL-6, and IL-1β profiles were analyzed with immunohistochemistry staining and the macrophage count with hematoxylin-eosin staining. The Nrf2 profile was increased after 5 days of treatment and the IL-1β profile was consistently decreased (p < 0.05). The IL-6 profile started to decrease after 5 days, while the macrophages increased after 3 and 5 days. No significant difference was found in TNF-α expression. DLS-CS proved effective in decreasing IL-6 and IL-1β profiles as well as increasing Nrf2 and macrophage migration in oral ulcers with diabetes mellitus.

Key words: distilled liquid smoke coconut shell, oral ulcer, diabetes mellitus, pro-inflammatory cytokines, macrophages

Similar Articles

Hyperglycemia in acute ischemic stroke: physiopathological and therapeutic complexity.
Ferrari F, Moretti A, Villa RF
Neural regeneration research. 2022; 17(2): 292-299

Purification of Cytoskeletal Proteins by Fast Protein Liquid Chromatography (FPLC) Using an ÄKTA Start System.
Gotesman M, Wang Y, Madasu SC, Mitchell CA
Methods in molecular biology (Clifton, N.J.). 2022; 2364(): 237-249

Delivery of Double-Stranded RNAs (dsRNAs) Produced by Escherichia coli HT115(DE3) for Nontransgenic RNAi-Based Insect Pest Management.
Taracena ML, Garcia Caffaro I, Paiva-Silva GO, Oliveira PL, Rendon PA, Dotson EM, Pennington PM
Methods in molecular biology (Clifton, N.J.). 2022; 2360(): 279-294

Determination of Bioenergetic Parameters in Mycobacterium ulcerans.
Thomas SS, Pethe K
Methods in molecular biology (Clifton, N.J.). 2022; 2387(): 219-230

Turbidity-Based MIC Assay and Characterization of Spontaneous Drug Resistant Mutants in Mycobacterium ulcerans.
Thomas SS, Kalia NP, Pethe K
Methods in molecular biology (Clifton, N.J.). 2022; 2387(): 209-217

Full-text options

Latest Statistics about COVID-19
• pubstat.org

Add your Article(s) to Indexes
• citeindex.org

Covid-19 Trends and Statistics
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.

ScopeMed Web Sites