In the present study, efforts were made to increase the enzyme production by modifying the nutritional conditions and pH of the fermentation medium. The results revealed that cellulase activity of 13.18U/ml was seen under appropriate cultural conditions by S.fungicidicus strain RPBS-A4 are better than the prior revealed strains. Notwithstanding these, some extra properties like external carbon source (maltose) and lower substrate fixation, less fermentation period for cellulase production showing the capability of the isolate to be utilized at profitable level in industries. An outline of the outcomes acquired demonstrates that solid state fermentation of agro processing waste deposits were appropriate to deliver minimal effort, high-esteem item i.e., cellulase by S.fungicidicus RPBS-A4. The ecological contamination caused by the disposal of cellulosic wastes, which are continuously added to the environment through the process of photosynthesis will also reduce by utilizing agro processing waste as a sole carbon hotspot for the generation of cellulase.
Key words: Cellulase, Streptomyces fungicidicus strain RPBS-A4, Agro processing waste (APW), Solid state fermentation
|