Thirty one yeasts isolated from sugarcane juice and process-sediments collected from different area of sugar factory were screened for ethanol production at 40oC.The isolates were belonged to 7 genera, namely Pichia, Ogataea, Millerozyma, Meyerozyma, Candida, Kluyveromyces and Saccharomyces based on phylogenetic analysis of D1/D2 region of the large subunit ribosomal (LSU) RNA gene. The majority of the isolates were identified as Pichia kudriavzevii (Group IB, 14 isolates), Ogataea polymorpha (Group II, 5 isolates), Kluyveromyces maxianus (Group VI, 5 isolates), Pichia manshurica (Group IA, 3 isolates),while the minor isolates were Candida tropicalis (Group V, 1 isolate), Millerozyma farinosa (Group III, 1 isolate), Meyerozyma caribbica (Group IV, 1 isolate) and Sacharomyces cereviseae (Group VII, 1 isolate).The isolates produced ethanol ranged from 2.97 to 57.10 g/L at 40oC. Isolates G3-9(1) and G3-3(1), identified as K. marxianus and S.cerevisiae showed high potential for ethanol production, 57.10±0.23 and 49.42±0.34 g/L, respectively.
Key words: Diversity, ethanol, Pichia, Kluyveromyces, Sacharomyces, thermotolerant yeasts
|
