Lactobacilli can be introduced as probiotics because of their health-promoting characteristics and non-pathogenic nature. This study aimed to perform the isolation, molecular identification, and probiotic characterization of Lactobacillus to find candidate strains isolated from unpasteurized milk of Cow and samples of young calves ileum with probiotic activity. Forty- two isolates were obtained. Of these, 5 Lactobacillus strains belonging to five species (L. paraplantarum strain S143, L. pentosus strain P2-3, L. plantarum strain J12, L. gasseri strain F. and L. reuteri strain IRT) were identified. They demonstrated remarkable results such as resistant to simulated gastric juices with pepsin and simulated small intestinal juice with pancreatin, susceptible to some antibiotics, good antimicrobial activity and strong adhesion to Caco-2 cell line that candidate them as potential probiotics. Moreover, the compatibility between the five selected starins was tested and there was no antagonism found between them.
Key words: probiotics -lactobacilli -antimicrobial activity - Caco-2 cell - antagonism
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