Comparative Study on Determination of Histamine Using HPLC and ELISA in Marine Fish and Control of Excessive Histamine in Fish Fillet by Natural Oils
Reyad R. Shawish, Zakaria Hassan, Walaa Abd-Elmegid.
Abstract
Our a complete of 60 samples of fresh marine fishes represented by Sardine, Saurus, Barboni and Chrysophyres were collected at different times from various fish markets in Alexandria governorate, Egypt and examined for determination of their levels of histamine by HPLC from one side and ELISA from the other side to study the accuracy of the two techniques. Results showed that all of specimens contained detectable value of histamine but the highest unacceptable sample in sardine fish and lowest unacceptable sample in Chrysophyres fish and there is no significant differences (P
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