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Original Article

AJVS. 2017; 54(1): 173-178


Genetic Diversity of Pasteurella Multocida Isolated from Different Animal Species

Samy A. Khalil, Ahmed A. Bkheet, Fayez A. Gad Allah.

Abstract
In this study, a total number of animal samples (583) were collected from cattle (170) , sheep (221) and goats (192) suffering from respiratory manifestations (freshly dead and slaughtered). Nasal swabs (169) were collected from cattle (43), sheep (67) and goats (59) . Blood samples (312) were collected from cattle (95) , sheep (112) and goats (105) and lung and spleen tissues (102) were collected from cattle (32) ,sheep (42) and goats (28) . Samples collected for bacteriological isolation and identification of P. multocida. After that confirmation of identification by using PM-PCR, and multiplex-PCR for detection of the virulence factors genes, DNA nucleotides sequencing of these genes were done for detection of the genetic diversity.
The prevalence of P.multocida isolated from all samples from Cattle, Sheep and Goats is 1.76%, 1.8%, 1.56% respectively. While, P. multocida not found in all blood samples collected from cattle, sheep and goat.
PM-PCR and multiplex-PCR confirmed presence of genes under examination.
DNA nucleotide sequencing and analysis of sequences obtained for the 5 genes under investigation Concluded that: P. multocida isolates had no genetic diversity of oma 87 gene, ptf A gene, phf A, tox A and nan B among cattle, sheep and goats.

Key words: Genetic diversity, Pasteurella multocida, virulence factors genes.


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