Selective Spectrophotometric Method for the Hydrocortisone Butyrate Quantitative Determination in Compounding Ointment in Presence of Nitrofural and Procaine Hydrochloride
Lesia P. Savchenko, Valentin O. Vrakin, Volodymyr O. Grudko, Tetiana V. Krutskikh, Volodymyr K. Yakovenko, Victoriya A. Georgiyants.
Abstract
Methods of sample preparation for the hydrocortisone butyrate excretion from compounding ointment were studied. The new procedure for its quantitative determination by spectrophotometry in the presence of nitrofural and procaine hydrochloride was developed during the research process. It is based on the hydrocortisone butyrate ability to interact with phenylhydrazyne with forming a phenylhydrazone, which has a maximum absorption at 412 nm. The developed method allows conducting its quantitative determination in the presence of other ointment components. The total uncertainty of analysis, the parameters of linearity, accuracy and precision were calculated. The recovery of hydrocortisone butyrate was found to be 100.82 %. The correlation coefficient was found to be 0.9995. The validation of the developed method proves its specificity and accuracy and allows recommending it for the hydrocortisone butyrate quantitative determination in the presence of procaine and nitrofural.
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