Diabetes mellitus, as one of metabolic disorder, can be characterized by postprandial hyperglycemia. An alpha-glucosidase inhibitor can be one of the choices treatment to prevent postprandial hyperglycemia. Garcinia lateriflora is one of natives plant in Indonesia that not much study for its alpha-glucosidase inhibitory activity. Therefore, this study aimed to evaluate and acquire the most active biologically fraction from leaves of G. lateriflora in inhibiting alpha-glucosidase. The ethyl acetate and methanol extract which exhibit the highest inhibition of alpha-glucosidase were separated by column chromatography and identified by thin layer chromatography, AlCl3 and H2SO4 spray reagent. Fraction EA13 from ethyl acetate and Me10 from methanol extract were the most active fraction from each extract with IC50 value 8,96 µg/ml and 18,52 µg/ml, respectively. Fraction EA13, which give highest inhibitory activity was further identified. It is showed that fraction EA13 reacted positively with AlCl3 and indicates the present of phenolic compounds.
Key words: alpha-glucosidase, column chromatography, Diabetes mellitus, Garcinia lateriflora, leaves, in vitro assay.
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