Optimization of biologically active substances extraction process from Potentilla reptans L. aerial parts
Vyara Borisova Lincheva, Nadezhda Traicheva Petkova, Ivan Georgiev Ivanov.
Abstract
Abstract:
European cinquefoil (Potentilla reptans L.) (Rosaceae) is very common in the Central Europe and Balkan Peninsula. Since ancient times the European cinquefoil have been traditionally used in herbal medicine for treatment of tooth ache, ulcers and inflammation of the throat, as well as certain forms of cancer, infections due to bacteria, fungi and viruses, diarrhea, diabetes mellitus and other ailments. Most species of order Potentilla contain hydrolysable tannins, proanthocyanidins, flavonoids and triterpens as bioactive substances. The aim of this study was to establish the best condition for extraction of biologically active substances from Potentilla reptans aerial part. The influence of the duration of the ultrasonic extraction and solvent system (ethanol-water) in different concentration ratio over the extraction process was studied. The optimal conditions for the extraction of biologically active substances from European cinquefoil were as follow 40% ethanol-water as solvent system and extraction time 45 min in ultrasound bath with frequency 35 kHz. Under these conditions the maximum values of total polyphenols content, total proanthocyanidins and total hydrolysable tannins (60.2 mg GAE/ g dw, 93.1 mg LE/100g dw, 101.3 mg TAE /g dw) were obtained.
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