Investigation, we focus to study the drought tolerance phylogenetic relationships among four Egyptian wheat species based on chloroplast gene rbcL and compared with other rbcL gene for wheat drought tolerance of the closer accessions data present in the NCBI-database. The evaluation of several parameters such as nucleotide frequencies, nucleotide substitution (r), and the estimated values of transition/transversion bias (R) indicated the presence of a wide divergence pattern of rbcL in Triticum aestivum L. UPGMA tree among the ten cultivars of Triticum aestivum L were formed to present a graphic demonstration of the patterns of divergences with rbcL sequence data using MEGA 7 software. Our phylogenetic tree was able to cluster the four wheat species from Egypt, all together with narrow relationships in a sister clade with others supported resolved the drought tolerance species into distinct branches among the Triticum aestivum L. Ongoing analyses of rbcL sequence analysis will allow us to assess the taxonomy and systematics of the different subgroups recovered with a good performance in explaining genetic drought tolerance diversity within and between, the four egyptian Triticum aestivum L. and related genera. Our results indicate that rbcL gene sequence analysis provides a well-defined tool to study drought tolerance species relationships within and among Triticum aestivum L, and can be successfully used in constructing reliable drought tolerance phylogenetic tree.
Key words: Egyptian Triticum aestivum L., drought tolerance, phylogenetic relationships, rbcL, ML, nucleotide substitution.
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