Carbendazim and copper oxychloride are two systemic fungicides used widely in control of fungal diseases in the field of agriculture. Although there is little information is available on the toxicity of these fungicides on human skin and liver. The study aimed to investigate the cytotoxic effect of carbendazim and copper oxychloride on HaCaT keratinocyte and hepatoma cell line HepG2 and correlate this toxicity caused by these two fungicides on human skin and liver. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphehyltetrazolium bromide) assay was used to determine the cell viability and proliferation. The results showed that these two fungicides showed concentration dependent cytotoxicity, upon incubation for 24 hours with concentration ranging 20-450 µg/ml. The cell viability of HaCaT cells decreased from 100% to 35.86% with increase in carbendazim concentration 20-450 µg/ml. Whereas HepG2 cells were sensitive towards Carbendazim treatment with the decrease in viability up to 30.98% at 450 μg/ml. Copper oxychloride was more lethal, causing total cell death of HaCaT cells at 350 μg/ml, and HepG2 at 450 μg/ml. The study unveils the cytotoxic effects of these fungicides on the skin as well as on the liver indicating the long term exposure to these compounds may lead to deleterious effects.
Key words: Carbendazim; Copper oxychloride; HaCaT; HepG2; MTT; Cytotoxicity.
|
