Development of alternative, less harmful and feasible sperm sexing procedures through swim-up technique are cost-effective and highly desirable for domestic animals, especially bulls. Hence, the study was planned to assess fresh and post-thaw sperm quality after dextran swim-up separation. Semen was collected from four bulls to evaluate sperm quality, and separated fractions were divided into two groups (10 and 15 min.) according to the time allowed for swim-up separation using dextran transition layer and later cryopreserved using Tris-based extender. It was observed that concentration of spermatozoa and progressive motility% significantly (P0.05) decreased in all fractions of two groups in comparison to fresh whole semen. We found significantly (P0.05) difference was observed in different parameters except motility% of Fraction 4 of two groups. Notably, there was no significant (P>0.05) difference found in kinematic parameters of fractioned semen collected at 10- and 15-minutes interval. After freezing and thawing, motility% and progressive motility% significantly (P
Key words: Spermatozoa, Swim-up, Cryopreservation
|
