Molecular characterization and antioxidant potential of Andean Chlorophytes from Ecuador
Juan Ortiz Tirado, Francisco Flores, Sebastián Maldonado, Raluka Mihae, Blanca Naranjo, Daysi Muñoz, Bangeppagari Manjunatha, Mariadoss Selvanayagam, Bugude Rajeswari.
Abstract
Microalgae are an important bio resource for the change of the productive matrix within the energy, agricultural and food sectors. In Ecuador, there are several studies that use native microalgae for bioremediation, food production and as alternative fuels. However, it is not common to identify this macrophyte at the species level, as well as the determination of nutritional and pharmaceutical properties. Classical identification of microalgae is based on their morphology and, in general, requires experienced taxonomists to achieve specific typifications. Molecular methods allow us to perform species-level identifications based on the amplification of multiple loci, where the sequences obtained can be compared to available public databases. Amplified DNA fragments show the presence of endemic species of nutraceutical interest and, together with chemical extraction processes, can provide reliable information for use in the agricultural industry. Under this condition, the present research with endemic species of the genus Chlorella sp., belonging to the Cayambe Coca Reserve with antioxidant potential and use for environmental remediation as functional feed for livestock farms.
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