The copper-containing metalloprotein tyrosinase catalyzes the oxidation of tyrosine in particular L-DOPA to L-Dopaquinone that generates brown pigments in the wounded tissues. The industrial demand for tyrosinase enzyme is increasing as they have a wide range of applications in the field of environmental, food, medicine, paper, pulp and textile industry. In the present investigation, tyrosinase was extracted from various plant sources and Dioscorea alata having a maximum enzyme activity was chosen. The different parts of plant material were taken for callus development and their enzyme activities were estimated. The enzyme activities of all calli were high comparing to the enzyme extracted from natural tuber tissue of D. alata making in-vitro culture as an alternative way for tyrosinase production. It is also evident that 2,4-D has the maximum callus development comparing to other plant growth regulators making it a perfect candidate for proceeding further with media optimization in future.
Key words: Yam, callus, picloram, 2,4-D, L-DOPA
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