Abstract

Biofilm formation is a major strategy of drug resistant bacteria to thrive and cause infection. This ever increasing drug resistance demands for new drugs and treatment. Our study investigated the potential of phytochemicals in inhibiting biofilm formation through screening of anti quorum sensing activity. Ethyl acetate extracts of leaves of Plumeria alba and Cynodon dactylon and methanol extract of leaves Pisonia alba were assayed to determine their ability to inhibit the in vitro biofilm formation. C.dacytlon extract showed an inhibition of 94.64±0.44% at 20 µg/mL concentrations against the bio film producing clinical isolate Pseudomonas aeruginosa. Whereas Plumeria alba and Pisonia alba extracts showed 90.30±0.27% and 88.25±0.82% inhibition activity respectively. C.dacytlon extract also exhibited considerable antioxidant activity of 65.42±0.787 through DPPH assay. Thus it indicates that these plants could be prospective agents in treating clinical pathologies through inhibition of cell communication.

Key words: Biofilm, Plumeria alba, Pisonia alba and Cynodon dactylon ,Pseudomonas aeruginosa, DPPH antioxidant activity