Abstract

Citrate capped silver nanoparticles (Ag NPs) was used as a colorimetric probe for the sensitive determination of six drugs belongs to fluoroquinolone family (Ciprofloxacin, Lomefloxacin, Ofloxacin, Pefloxacin, Levofloxacin and Moxifloxacin). The interaction between the studied fluoroquinolones and citrate-capped Ag NPs resulted in the appearance of new surface plasmon resonance (SPR) peaks measured in the range of 598 – 696 nm. Under the optimized conditions, good linear relationships (R2 = 0.9973–0.9991) were obtained in the range of 1.50–10.25 , 2.0–10.25, 2.0–10.50, 2.0–10.50, 1.5–10.0 and 2.0–11.0 µg/mL for Ciprofloxacin, Ofloxacin, Levofloxacin, Moxifloxacin, Lomefloxacin and Pefloxacin, respectively. The analytical performance of the method was fully validated, and the results were satisfactory. Different pharmaceutical dosage forms containing the cited drugs were successfully analyzed by the suggested method with high degree of accuracy and precision. The proposed method is environmentally safe as it use water as a solvent and does not involve solvent extraction.

Key words: Surface plasmon resonance spectroscopy; Fluoroquinolones; Silver nanoparticles; Pharmaceutical analysis