BLAD is an Autosomal genetic disorder mainly in Holstein and its crosses are major concern worldwide as heterozygous (carrier) looks normal and therefore, used for breeding programmes. During the last one and half years, blood samples were collected in the heparinized blood collecting tubes from 570 dairy bulls including 11 HF and 81 HF crossbred bulls for investigation of heterozygous/carrier animals for various genetic disorders, including Bovine Leukocyte Adhesion Deficiency Syndrome. The DNA extraction, PCR and RFLP were performed to find out carrier animals. PCR-RFLP techniques exhibited a polymorphism in CD18 gene in two Holstein crossbred bulls, which were confirmed by sequencing. The percentage of recessive allele in Holstein and Holstein crossbred samples was calculated to be 2.17. Rest all animals were found normal.
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