Abstract

Aim: This study aimed to evaluate the long-term stability of cattle plasma and serum, measuring changes in the concentration of some biochemical analytes under different temperatures and time conditions of storage, and assessing the potential clinical impact of these changes.
Methods: A total of 7 biochemical analytes in the serum and plasma of seventy-four adult cattle were examined following storage. After determining the baseline measurements (D0), the serum and plasma of each cattle were aliquoted and stored at 4°C and −20°C for 15, 45, 75, 105, 135, 165, and 180 days and then analyzed for stability. The results were compared with the initial analysis measurements obtained from fresh samples. The mean concentrations of biochemical analytes in different storage conditions were compared to the initial concentrations (D0) using a paired t-test and compared to reference values to evaluate the potential clinical impact.
Results: The glucose, creatinine, and cholesterol were stable under all conditions. Glucose and aspartate aminotransferase (AST) concentration in serum vs. plasma did show significant differences (p

Key words: Cattle, plasma, serum, stability, storage, and temperature.