The study investigates the use and dosage of clove oil as aesthetic in stress African catfish. Fish were soaked in a water containing five concentrations were made at 65, 75, 85, 95, and 105 mg/L and the blood were taken and haematology, plasma biochemistry, and serology parameters were examined. Descriptive, ANOVA and regression analysis employed to analysed the data. The results showed that concentrations of 95 mg/l and 105 mg/l of E. caryophyllata oil induced mean number of fish of 8.67 ± 1.53 and 9.67 ± 0.58, respectively. The fish induced with 65, 75, and 85 mg/l had highest number of fish recovery of 10.00 ± 0.00 while concentration of 105 mg/l had the least (5.67 ± 1.53) number of fish recovery at 3 minutes. Induction relationship and the concentration of oil (y = 57.91 - 27.45x, R² = 0.907) while the recovery time relationship and the concentration of oil (y = 50.08 - 29.41x, R² = 0.921). The fish from the best concentration (95mg/l) was used for blood examination. Haematological parameter of the C. gariepinus juveniles anesthetized with E. caryophyllata oil at 95 mg/l. PVC (27.00 ± 1.00%), Hb (8.73 ± 0.31 dl), RBC (3.25 ± 0.50 × 106/ µl), WBC (18.72 ± 0.44 × 106 µl), Lymph (71.33 ± 1.53%), Hetero (22.33 ± 1.53%), Eosinophil (3.00 ± 0.00%), Monocytes (3.33 ± 0.58%), MCV (84.20 ± 10.44 FI), MCH (27.24 ± 3.62 Pg), and MCHC (32.36 ± 0.95 %) of the anesthetized fish is similar to the non-anesthetized C. gariepinus juveniles. Total protein (5.47 ± 0.12 g/dl), albumin (1.53 ± 0.06 g/dl), globumin (3.93 ± 0.15 g/l) and A: G ratio (0.33 ± 0.06) were observed in the fish blood. Apart from AST and ALP where anesthetized fish had 194.17 ± 10.11 µl and 232.17 ± 63.64 µl respectively which is higher than non-anesthetized. ALT, Urea, glucose, and cholesterol were within the same and acceptable range of the normal C. gariepinus juvenile blood enzyme indices. E. caryophyllata oil is an effective anaesthetic material and could be used in C. gariepinus farming and research. The effective dose from this study is 95 mg/l without altering blood indices.
Key words: Eugenia cayrophyllata Oil, Anaesthetic, Clarias gariepinus Juveniles
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