Chlorella vulgaris and Chlorella salina were successively cultivated in sewage and different mixtures of water samples for 10 days to investigate the changes in growth and some physiological parameters. Optical density, dry mass, photosynthetic pigments (Chl.a, Chl.b, and Carotenoid) and organic solutes (soluble sugars, soluble proteins and total free amino acids) of both algal species were significantly decreased in response to sewage and different mixtures of water samples. The cultivation of the two investigated Chlorella in different types of water stimulated the biosynthesis of some different amino acids, whereas caused inhibition of others within the cells. On the other hand, there was marked increase in the activity of catalase (CAT), Peroxidase (POD) and ascorbate peroxidase (APX), as well as malondialdehyde (MDA) contents of C. vulgaris and C. salina cells under these treatments. The data showed that C. salina was more sensitive to sewage and different mixtures of water than C. vulgaris.
Key words: Amino acids; Ascorbate peroxidase; Catalase; Chlorella; Malondialdehyde; Peroxidase; Water samples.
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