Expression profiles of the Tau-associated genes GSk3β, CAPN1, and CDK5R1 in the brain cortex of aged female cynomolgus monkeys with cognitive impairment
Huda S. Darusman, Lis Rosmanah, Sela S. Mariya, Uus Saepuloh, Yuliana Yuliana, Jann Hau.
Background:
Alzheimer's disease (AD) is characterized by the buildup and aggregation of misfolded proteins in the brain, including amyloid-β (Aβ) and hyperphosphorylated tau. The hyperphosphorylation state of Tau protein plays an important role in the development of AD. Our previous studies developed and characterized the cynomolgus monkey as a spontaneous animal model of AD.
Aim:
We demonstrated the validity of the model through experimental investigations of the relationships between cognitive decline and the neuro-pathy of AD. There is, however, little information about the expression actvity of hyperphosphorylated tau related genes in various brain areas in the in cynomolgus monkey spontaneous AD model.
Methods:
In the present study, total RNA was extracted from archived cortex and hippocampus tissues from the brains of two groups of cynomolgus monkeys, adult (10-12 years old, n=5) and aged (> 20 years old, n=4). The expression of the tau-protein-associated genes GSK3β, CAPN1, and CDK5R1 was evaluated using RT-qPCR technique
Results:
The expression of all three genes was increased up to five fold in the brain cortical area of the aged subjects compared to the adults
Conclusion:
The results add weight to the utility of cynomolgus macaques as a valid spontaneous model in translational preclinical research involving studies of the effect of aging on the formation of hyperphosphorylated tau protein, which causes AD-related lesions in the brain.
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