The present investigation explores the green synthesis of titanium dioxide nanoparticles (NPs) using an aqueous extract of Ulva lactuca, a medicinally important marine macroalgae. Characterization of UL-TiO2NPs showed an intense peak at 371 nm. Fourier transform-infrared spectroscopy analysis showed the presence of functional groups such as hydroxyls, carbonyls, methylene, aliphatic and aromatic hydrocarbons, amines, and ethers. X-ray diffraction confirmed the crystalline nature of the biosynthesized UL-TiO2NPs with sizes in the range of 20 and 50 nm. Scanning electron microscope analysis emphasized the morphology of TiO2NPs to be spherical-shaped with minimal clustering. Antibacterial activity indicated profound inhibitory zones against clinical isolates (12–19 mm) rather than against microbial type culture collection bacterial pathogens (9–14 mm). The anti-inflammatory activity of the UL-TiO2NPs was affirmed by a remarkable reduction (22%–78%) of protein denaturation. Anticancer activity of UL-TiO2NPs against oral oral carcinoma cell line carcinoma cells showed a notable reduction in viability of cells (94.31%–17.4%) with respect to an increase in concentrations and recorded an IC50 value of 28.74 μg/ml. Thus, the distinct properties of biologically synthesized titanium dioxide NPs imply that they could be efficiently utilized as a nano-antibiotic coating on the surface of healthcare devices and as a drug carrier in anticancer studies.
Key words: Ulva lactuca, TiO2NPs, Antibacterial, Anti-inflammatory, Cytotoxicity
|
