Background:
Macrocyclic lactones (MLs) are pharmaceutical compounds extensively utilised in the management of Rhipicephalus microplus tick infestations in bovine populations. It is of paramount importance to prevent or delay the development of drug resistance to ML. In vitro techniques are validated by FAO and can serve as an orientative diagnosis of the resistance developed in field conditions. Diluent selection must be considered when sensitivity on field strains is beeing studied. The Syringes Immersion Test (SIT) is a modification of the larval immersion test where syringes are used seeking to reduce the workload.
Aim:
Study the interchangeability of two diluents in the diagnosis of sensitivity to MLs on Rhipicephalus microplus larvae using the SIT technique.
Methods:
Dose-response curves were adjusted using SIT with MLs, on different diluentes (Acetone [ACT] and Dimethyl sulfoxide [DMSO]) on Mozo strain (standard susceptible strain). Slope, potency and discriminating concentration were estimated for each drug on both
diluents. A 4-parameter log-logistic model was applied for model fitting. Ratio between estimated parameters was used to compare results. Field strains were tested on both diluents for each drug, using the discriminating concentration estimated for Mozo strain.
Results:
For the Mozo strain, dose-response models were adjusted for each drug on both diluents using SIT. Ivermectin (IVM) and doramectin (DRM) showed no significant difference for slope when comparing diluents (p > 0.05); Moxidectin (MOX) presents a higher sensitivity for DMSO vs IVM (p
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
The articles in Bibliomed are open access articles licensed under Creative Commons Attribution 4.0 International License (CC BY), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
We use cookies and other tracking technologies to work properly, to analyze our website traffic, and to understand where our visitors are coming from. More InfoGot It!
