L-methioninase has been purified 2.55-fold from the crude extract of Streptomyces sp. DMMMH4. The purification procedure was carried out by heat treatment and gel filtration on Sephadex G-200 column chromatography. SDS-PAGE electrophoresis showed a migrating protein band molecular mass of 48 kDa. The kinetic properties determined for the purified enzyme displayed optimum activity at 70OC and thermal stability was 70OC for 30 min. The enzyme activity showed maximum activity at pH 6 using acetate buffer 0.05M and was relatively stable across a broad range of pH values (5.5-8 pH). The enzyme strongly inhibited by Cr+2, Fe+2, Ni+2, Cd+2, PMSF, β-mercaptoethanol and SDS while Hg+2,Cu+2 and iodoacetate completely inhibited the enzyme activity at a final concentration of 10mM. The purified enzyme exhibited a Km of 0.7, 0.15 and 0.25 mM for L-methionine, DL-ethionine and L-cystine respectively. Cytotoxicity test demonstrate that enzyme was active against liver HepG2, breast MCF-7, lung A549, prostate PC3 and colon HCT116 cancer cell lines and has negligible toxicity toward a normal melanocyte cell line HFB4 .
Key words: L-methioninase, Streptomyces spDMMMH4, Purification, Thermo stable, anticancer.
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