Abstract

Objective: To determine the association of elevated levels of Monocyte Chemo attractant Protein-1 (MCP-1) in saliva and gingival crevicular fluid of severely affected chronic periodontitis patients with well-established progression and acute precipitation of cardiovascular diseases.
Methodology: This case-control study included 100 subject between the ages of 35-68 years. Cases with severe periodontitis along with horizontal alveolar bone loss (grade 4) having cardiac disease were selected and individuals with periodontitis without cardiac diseases were opted as controls. A structured questionnaire was used to collect detailed demographics, cardiovascular, medical, and family history of the patients. Grading of alveolar bone loss, periodontal and gingival status, probing depth, gingival index, plaque index and clinical attachment level were recorded. Gingival crevicular fluid and salivary sample were extruded for the biomarker and quantified in a solid phase, sandwich technique using Elisa kit.
Result: Periodontal assessment in cases revealed that 4% had moderate and 96% had severe form of periodontitis with 24% cases had grade-3 and 76% cases had grade-4 horizontal alveolar bone loss. The chi square test showed significant differences in the proportions (p

Key words: Periodontitis, monocyte chemoattractant protein-1, gingival crevicular fluid, plaque and gingival index, clinical attachment.