Background:
Disease prevention in cultivating ranchu goldfish (Carassius auratus) can be prevented by giving immunostimulants in the food. One immunostimulant can come from herbs such as calabash fruit (Crescentia cujete L.).
Aim:
This study aims to analyze the potential of calabash fruit extract (BFE) on the hematological profile of ranchu goldfish.
Methods:
This study used 30 ranchu goldfish, which were divided into six treatments, as follows: P1 = standard food; P2 = food + 0.5% CMC Na; P3 = food + 0.1% POWER C®; P4 = food + 0.5% BFE; P5 = food + 0.3% BFE; and P6 = food + 0.6% BFE. The treatment was performed for 28 days with a feeding frequency of 2 times a day. On the 28th day, fish performance was measured. The blood was collected for hematology and immunohistochemistry against antibody anti-COX-2 and CD4+. The data were statistically analyzed using SPSS with confidence level at 95%.
Results:
The group P6 indicated the highest growth performance compared to the other groups (p
Key words: Calabash fruit extract, CD4+, COX-2, Hematology, Ranchu goldfish
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