Background:
Regenerative endodontics' primary objective is to establish a favorable environment in the root canal by removing infection, providing a sturdy scaffold, and sealing the apical end of the tooth tightly. These actions should promote pulp regeneration and root development.
Aim:
This study evaluated histologically the regenerative potential of injectable hyaluronic acid (HA) hydrogel or collagen with blood clot as scaffolds during revascularization of immature necrotic dog’s teeth.
Methods:
Sixteen permanent immature necrotic premolars with 32 roots were chosen from two 4-5-month-old mongrel dogs. Out of the 32 roots, 24 roots were sealed for two weeks after being cleaned with CaOH2. According to the treatment protocol, the 24 cleaned roots were randomly assigned to one of three treatment groups (each with 8 roots): blood clot + HA group, blood clot + collagen group, and blood clot group. The control group consisted of the eight infected roots that remained untreated. Three months after the surgery, the assessment of tissue ingrowth in the pulp cavity took into account the kind of cellular components, the intercellular matrix, angiogenesis, and the occurrence of any hard tissue formation. The pulp's capacity for regeneration was described descriptively, taking into account the type of regenerated tissue, the root's apical closure, and any potential periodontal and periapical histological alterations. Semi-quantitative analysis was used to assess the degree of pulp tissue regeneration. Tukey's post hoc test was used after a two-way ANOVA for statistical analysis of all the data.
Results:
When comparing the treated groups to the control group, a significant increase in tissue ingrowth and a significant decrease in the periapical inflammatory reaction were noted (P
Key words: Dental pulps, Periapical pathosis, Regenerative endodontic, Revascularization, Scaffolds
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