Abstract

Objective: To determine Eugenol’s anti-cancer potential in Chronic Myeloid Leukemia concentrating at anti-proliferative effects on human leukemia HL-60 cells.
Methodology: This study included culturing HL-60 cells in pre-formulated commercially available Dulbecco's Modified Eagle Medium (DMEM) for HL-60 cell line and its treatment with serial concentrations of Eugenol for calculation of subsequent IC50 values via MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay. Anti-proliferative activity was confirmed via Nitric Oxide (NO) releasing Assay.
Results: For HL-60 cells, the IC50 of Eugenol (10.1uM) showed persistent peaks of NO levels confirming its anti-proliferative potential. Strongest growth inhibitory activity was seen in the peak time of 100-120 min.
Conclusion: Eugenol was a strong anti-proliferative phytochemical agent leading to therapeutic effects against HL-60 cells. Further studies would help in identifying key molecular intricacies by which Eugenol exhibits diversified Anti-cancer potential.

Key words: Eugenol, Pro-apoptotic effects, anti-cancer mechanisms, HL-60 cells, natural chemotherapeutics.