Background:
Mitochondrial genomes play a key role in molecular biology research by providing essential information about evolutionary links, population history, and genetic diversity.
Aim:
The aim of this investigation was to produce partial sequence of 12S rRNA and 16S rRNA genes, as well as complete sequence of tRNA-Val gene in some species of family Labridae.
Methods:
Five species of labrid fishes (Oxycheilinus digramma, Cheilio inermis, Epibulus insidiator, Coris aygula and Gomphosus caeruleus) belong to Family Labridae were collected from the Red Sea, thereafter, taken to a lab for morphological identification in accordance with Randall (1982). Using forward and reverse primers (Wang, 2000), genome DNA was amplified through polymerase chain reaction.
Results:
The tRNA-Val gene's entire sequence, the 12S rRNA gene's partial sequence, and the 16S rRNA gene's partial sequence were all submitted to GenBank/NCBI with accession numbers (PP962382.1 - PP962386.1). The sequences' outcomes showed that the average A+T values were higher than the C+G values.
Conclusion:
The partial sequences of 12S RNA and 16S RNA, and the whole sequence of the tRNA-Val gene, were arranged that, the 12S RNA and 16S RNA have been distinguished by the tRNA-Val gene.
Key words: Mitochondrial 12S rRNA gene, 16S rRNA gene, tRNA-Val gene, Labridae
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