Background: Unlike mammals, zebrafish display a highly regenerative potential in response to cellular injury. Hence, it has gained much attention as a viable model for the study of regeneration. Studies on zebrafish regeneration frequently concentrate on adult tissues such as the heart, brain, retina, caudal fin, and spinal cord.
Methods: This study was conducted under the PRISMA guidelines. The search for primary research articles was conducted using PubMed, Web of Science, and Mendeley. We used the latest update of Microsoft Office Excel. Of the total 1,158 results, 1,066 were dropped according to the criteria for exclusion. The selected results included previously published and unpublished studies on cardiac cell regeneration in zebrafish from 2012 to 2022.
Results: 90 studies met the inclusion criteria. Out of these, 43 used the apex resection method, 36 used cryoinjury, and 16 used genetic amputation. All methods used were based on selected heart sections, not the whole heart. The primary evaluation technique used in the included studies was histology, either alone or in combination with other methods. Acid Fuchsin Orange G, Masson’s Trichrome, Hematoxylin/Eosin, immunofluorescence, and in situ hybridization were the main histological techniques employed to assess heart regrowth and regeneration.
Conclusion: This study may have a risk of bias due to the qualitative and quantitative data that was selected. Further research can help understand and utilize zebrafish regeneration genes in humans.
Key words: Zebrafish , cardiac cell, regeneration , danio rerio ,apex resection
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