Objective: This study was designed to investigate the antiapoptotic and improving effects of aminoguanidine on the histological alterations in liver in streptozotocin (STZ)-induced diabetic rat model.
Material and Methods: 32 male Sprague Dawley rats were divided into the following 4 groups: Control, Aminoguanidine (AMG), Streptozotocin (STZ), Streptozotocin + Aminoguanidine (STZ+AMG). The animals in group STZ and STZ+AMG were made diabetic by intraperitoneal injection of streptozotocin 45 mg/kg. Histochemical and immunohistochemical staining methods were applied to sections obtained from paraffin blocks and preparations were examined by using Leica DFC-280 light microscope.
Results: The sections taken from the control and AMG groups were normal in histological appearance. However, multifocal nodules containing inflammatory cells were observed in the diabetic group. Moreover necrotic hepatocytes and hemorrhagic areas were observed among intact hepatocyte of lobul in the STZ group. Glycogen loss was observed in the hepatocytes localized at the periphery of lobule. In addition, the numbers of cells with positive staining by caspase-3 were significantly increased. On the other hand, it was seen that, in the groups administered with STZ, histological injury in the liver was attenuated by the administration of AMG. Moreover, it was found that the number of caspase-3 positive cells was significantly decreased in the STZ+AMG group.
Conclusion: We concluded that chronic aminoguanidine administration reduced liver injury in STZ- induced diabetic rats. Thus, we suggest that aminoguanidine may be used to prevent the development of diabetic liver damage.
Key Words: Aminoguanidine; Diabetes mellitus; Streptozotocin; Rat.
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