Urinary system infection is a serious health problem that effects millions of people each year. Along with this, the
rapid development of antibiotic resistance observed in the Enterobacteriaceae and Escherichia coli species which are
responsible for the majority of these infections constitutes a serious problem in its treatment. Thus the objective
of this study is to identify the susceptibility of the Gram-negative bacteria isolated from urine samples to 12
different antibiotics (ampicillin, ampicillin/sulbactam, amoxicillin/clavulanic acid, sefalotin, cefoxitin,
cefoperazone, gentamicin, amikacin, tobramycin, trimethoprim/sulfamethoxazole, ciprofloxacin and imipenem).
In identification of the isolated bacteria api 20E (BioMerieux) has been used together with the known
conventional methods. Antibiotic susceptibility is investigated by Kirby-Bauer disc diffusion method.
Totally 91 isolated bacteria were examined. These are identified as follows: 42 as Escherichia coli (462%); 18 as
Klebsiella pneumoniae (19.8%); 13 as Enterobacter cloacae (14.2%); 11 as Proteus vulgaris (12.1%) and 7 as Pseudomonas
aeruginosa (7.7%).
During our studies none of the examinated strains showed resistance to amikacin and imipenem, but high level of
susceptibility to gentamicin and tobramycin (57.0-100%) was observed. Although high resistance to ampicillin,
ampicillin/sulbactam and amoxicillin/clavulanic acid (69.0-100%) was identified in all the strains, the high
increase in the resistance to trimethoprim-sulfamethoxazole (38.9-100%) and ciprofloxacin (11.9-100%) was far
more considerable. Among the cephalosporins resistance to cefalotin was very high (92.8-100%) while the
minimum resistance was identified to cefoperazone (16.7-57.1%).
Key Words: Urinary System Infection, Gram-Negative Bacteria, Antimicrobial Susceptibility.
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