The advantages and disadvantages of the native, lugol and trichrome methods used for the diagnosis of intestinal
protozoa were investigated.
500 stool samples were examined with saline(native), lugol and trichrome staining methods.
Of the stool samples analysed by native and lugol staining methods, G. intestinalis in 26 (5.2%), E. histolytica in 11
(2.2%), E. coli in 9 (1.8%), T. intestinalis in 10 (2%), B. hominis in 3 (0.6%), E. nana in 1 (0.2%), E. hartmanni in 1
(0.2%) and C. mesnilii in 1 (0.2%) were detected, whereas in the case of trichrome staining, G. intestinalis in 31
(6.2%), E. histolytica in 14 (2.8%), E. coli in 11 (2.2%), B. hominis in 4 (0.8%), E. nana in 2 (0.4%), E. hartmanni in 1
(0.2%) and were determined. We were unable to detect T. intestinalis and C. mesnilii by this method.
In conclusion, in routine parasitological stool examinations, especially in amebic cases, trichrome staining along
with native- lugol method may provide great benefit in the detection and diagnosis of the agent.
Key Words: Diarrhea, Native, Lugol, Trichrome Staining, Protozoa
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