Aim: The aim of this study is to investigate the effects of methylene blue (MB) on NO production, antioxidant
status and lipid peroxidation in lung injury during the different stages of sepsis in rats.
Material and methods:Sixty Sprague Dawley rats weighing 200-230 g, were randomly divided into 3 groups. The rats
of the first group were sham operated (control, group C); the second group, sepsis (group S); the third group
received MB (25mg/kg, i.p.), MBS (sepsis+MB). Sepsis was induced by caecal ligation and puncture method. Each
group was randomly subdivided into two subgroups (early and late sepsis) consisting of 10 rats which were
sacrificed at 9 or 18 h after surgical procedure. In the lung tissue, total nitrite/nitrate (NOX), superoxide dismutase
(SOD), catalyse (CAT), glutathion peroxidase (GSH-PX) and malondialdehyde (MDA) levels were measured.
Damage to the lung tissue was graded by a pathologist on a scale of 1 (best) to 4 (worst).
Results: In group MBS, CAT levels increased significantly both in early and late sepsis groups, whereas, SOD and
GSH-PX levels increased significantly only in early sepsis groups compared to group S. Methylene blue, significantly
decreased NOx and MDA levels in both early and late sepsis groups compared to group S (p
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