Abstract

 

Objective: This study was designed to detect and evaluate the histochemical and immunohistochemical alterations in rat kidney histology following streptozotocin (STZ)-induced and fructose-induced experimental diabetes.

Material and Methods: Fifteen male Sprague-Dawley adult rats were divided into three groups as control, STZ and fructose groups. STZ group rats received a single dose of STZ (45mg/kg) intraperitoneally. Fructose group rats were fed by tap water containing 10 % D-fructose for 8 weeks. At the end of eight weeks rats were killed, left kidneys were removed. Following routine tissue process, kidneys were embedded in paraffin. Histochemical and immunohistochemical stains were applied and the specimens examined by light microscope.

Results: In both STZ and fructose groups histological changes were observad in the cortex. Increase of Basal membrane thickness in glomerule capillary, mesangial matrix, thickness in parietal layer of Bowman’s capsule and rare tubular basal membrane thickness were detected in both groups. In fructose group arteriol walls also showed increased thickness.

Conclusion: We concluded that both STZ and fructose induced experimental diabetes led to similar findings in rat kidneys and these findings probably occur as direct and/or indirect results of hyperglisemia.

 

Key Words: Experimental Diabetes, Rat, Kidney, Light Microscopy.