Physalis angulata L fruit extract is a promising natural substance with immunomodulatory properties. Therefore, this study aimed to characterize and investigate the immunostimulatory effect of P. angulata L fruit extract using hematological parameters and nitric oxide (NO) production. Fruit extract was characterized through standardization, LC-ESI-MS analysis, and determination of total phenolic content. In vivo immunostimulatory effect tests were conducted on Wistar rats using hematological parameters. Meanwhile, an in vitro test was performed on RAW 264.7 cells through cell viability and NO assays. LC-ESI-MS analysis showed 16 secondary metabolite compounds in P. angulata L fruit extract, and the total phenolic content was 2.895 mg/g gallic acid. Additionally, an in vivo immunostimulatory effect test identified that a 300 mg/70 kg BW dose of fruit extract increased the levels of leukocytes, lymphocytes, monocytes, and granulocytes in male Wistar rats. In vitro test results showed that cell viability induced by P. angulata L fruit extract averaged above 100% to a concentration of 500 ppm. Furthermore, NO production in cells administered with extract was higher compared to other treatments. In conclusion, P. angulata L fruit extract could enhance the immune system, suggesting the potential for development as a natural immunostimulant.
Key words: Physalis angulata L, LC-ESI-MS, Immunostimulant, Nitric Oxide, RAW 264.7 cells
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