Viruses cause infectious diseases in humans, ranging from mild to life-threatening. Early and accurate diagnosis is essential for timely treatment and prevention of complications. Diagnostic methods typically rely on detecting viral genetic material, proteins, or the host’s immune response, depending on the infection stage. Monoclonal antibodies, known for their specificity and high affinity, are widely used for diagnosing viral infections by enabling precise and sensitive detection of viral proteins. This review explores the applications of monoclonal antibody-based methods for detecting viral infections, emphasizing their pivotal role as sensitive detection reagents. These methods are crucial for developing robust immunodiagnostic assays capable of diagnosing various viral diseases in human, animal, and plant hosts, with special emphasis on SARS-CoV-2. The review also explores various assays that employ monoclonal antibodies for diagnostic purposes and the technologies used for their production. Understanding the principles and applications of monoclonal antibodies in diagnosing viral diseases is essential for implementing effective public health interventions and preventing future pandemics.
Key words: Hybridoma, Infectious Diseases, Immunodiagnosis, Phage Display, Point-of-care, Virus
|
