Abstract

Background:
The preservation of semen quality and kinematic characteristics during cryopreservation is crucial for the reproductive success and genetic management of livestock, particularly in Bali bulls.
Aim:
This study aimed to investigate the effect of adding purified green tea extract antioxidant Epigallocatechin-3-gallate (EGCG) in tris egg yolk diluent on the quality and kinematic characteristics of frozen semen from Bali bulls.
Methods:
Fresh and frozen semen samples were obtained from Bali bull and divided into four different treatment groups. P0 contained semen samples + diluent, while P1 to P3 consisted of semen samples + diluent supplemented with EGCG levels of 0.1 mg/100 mL, 0.15 mg/100 mL, and 0.2 mg/100 mL, respectively. Data were analyzed using One-way ANOVA and followed by Duncan's test if significant differences were found (p

Key words: Bali bull, EGCG, Kinematics, Livestock and gene bank, Sperm quality